Review



2x qiagen multiplex pcr plus master mix  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Qiagen 2x qiagen multiplex pcr plus master mix
    2x Qiagen Multiplex Pcr Plus Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+plus+kit/QIAGEN+Multiplex+PCR+Plus+Kit+x+%E2%80%8B%E2%80%8B100u/pmc13639227-78-6-7
    Average 94 stars, based on 52 article reviews
    2x qiagen multiplex pcr plus master mix - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Multiplex Assay:

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.
    Article Snippet: Total RNA was extracted from the brain (cerebral cortex), liver, or skin of B6J or MSM using Trizol reagent (Invitrogen) and treated with DNase I (Takara Bio). cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen) with oligo(dT) primer or random hexamer. .. We used LA Taq with GC buffers (Takara Bio) or Multiplex PCR Plus Kit with Q-solution (Qiagen) for PCR and always used a temperature gradient to optimize annealing. ..

    Article Title: Alterations in epigenetic marks and expression of genes related to stress regulation: an exploratory study among newborns after fetal repair of spina bifida aperta
    Article Snippet: 150–300 ng of total RNA were reverse transcribed into cDNA with the Whole Transcriptome Amplification Kit (WTA2, Merck/Sigma-Aldrich) and purified with NucleoSpin Gel and PCR Clean-up (740611.50; Macherey-Nagel, Switzerland). .. Pre-amplification with the Qiagen Multiplex PCR Plus Kit (206152) of the cDNA was conducted. .. Gene expression analysis of NR3C1 and FKBP5 was performed in triplicate (1 μL PreAmp cDNA) using QuantiNova SYBR Green detection (Qiagen, Hombrechtikon, Switzerland) and normalized with five reference genes ( RPL13a , PPIA , GAPDH , 18S , ACTB ).

    Article Title: Signals of corresponding genetic diversity loss in four warbler species exhibiting regional or range-wide declines.
    Article Snippet: .. First round PCR was performed using the Qiagen Multiplex PCR Plus Kit (206152) with 0.3ul of the above primer mix and 2 - 50 ng of DNA in a final volume of 11ul. ..

    Article Title: Signals of corresponding genetic diversity loss in four warbler species exhibiting regional or range-wide declines
    Article Snippet: .. First round PCR was performed using the Qiagen Multiplex PCR Plus Kit (206,152) with 0.3 μl of the above primer mix and 2–50 ng of DNA in a final volume of 11 μl. .. Initial denaturation at 95 °C for 5 min was followed with 30 cycles of 95 °C for 30 s, 62 °C for 90 s, and 72 °C for 30 s, and a final extension step of 68 °C for 10 min. A tenfold dilution of these products was used as the template for a second round of PCR to add the Nextera i5 and i7 index primers.

    Article Title: Optimal Environmental DNA Sampling Strategy for Comprehensively Assessing Biodiversity in Rice Paddy Fields
    Article Snippet: In the 1st PCR, the 5′ termini of the above primers had Illumina Rd1 and Rd2 sequences. .. PCR with fwhF2/EPTDr2n used the multiplex PCR Plus kit (Qiagen), with a thermal profile of 95°C for 5 min; a touchdown of 10 cycles of 95°C for 30 s, 64 to 55°C (−1°C each cycle) for 90 s, and 72°C for 30 s; 25 cycles of 95°C for 30 s, 54°C for 90 s, and 72°C for 30 s; and a final 68°C for 10 min. PCR with MiFish- U and MiBird- U used KAPA HiFi Ready Mix HS FIGURE 1 | Study paddies and eDNA sampling positions. (A) Location of paddies (pale blue rectangles, A–G). ..

    Article Title: Food poisoning from raw horse meat contaminated with Shiga toxin-producing Escherichia coli O157 linked to nationwide spread of closely related strains, Japan, 2023
    Article Snippet: The PCR conditions were as follows: 95°C for 2 min; 35 cycles of 95°C for 30 s, 60°C for 90 s, and 72°C for 60 s; with final extension at 72°C for 10 min. PCR products were separated using a SeqStudio Genetic Analyzer (ThermoFisher Scientific). .. At the NIID, the Qiagen Multiplex PCR Plus Kit (Qiagen) was also used for PCR amplification, and an Applied Biosystems 3500xl Genetic Analyzer (ThermoFisher Scientific) was used for the separation of PCR products. ..

    Article Title: Food poisoning from raw horse meat contaminated with Shiga toxin-producing Escherichia coli O157 linked to nationwide spread of closely related strains, Japan, 2023.
    Article Snippet: The PCR conditions were as follows: 95°C for 2 min; 35 cycles of 95°C for 30 s, 60°C for 90 s, and 72°C for 60 s; with final extension at 72°C for 10 min. PCR products were separated using a SeqStudio Genetic Analyzer (ThermoFisher Scientific). .. At the NIID, the Qiagen Multiplex PCR Plus Kit (Qiagen) was also used for PCR amplification, and an Applied Biosystems 3500xl Genetic Analyzer (ThermoFisher Scientific) was used for the separation of PCR products. ..

    Article Title: Novel Y-STRs with elevated mutation rates further improve male relative differentiation.
    Article Snippet: .. PCRs were performed using the Qiagen Multiplex PCR Plus Kit with the following reaction mix: 5 μL Multiplex PCR Master Mix (2 ×), 1 μL primer mix (forward and reverse primers, 10 μM each), 2 μL Q- Solution, 1 μL RNase-free water, and 1 μL template DNA (10 ng/μL). ..

    Polymerase Chain Reaction:

    Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.
    Article Snippet: Total RNA was extracted from the brain (cerebral cortex), liver, or skin of B6J or MSM using Trizol reagent (Invitrogen) and treated with DNase I (Takara Bio). cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen) with oligo(dT) primer or random hexamer. .. We used LA Taq with GC buffers (Takara Bio) or Multiplex PCR Plus Kit with Q-solution (Qiagen) for PCR and always used a temperature gradient to optimize annealing. ..

    Article Title: Alterations in epigenetic marks and expression of genes related to stress regulation: an exploratory study among newborns after fetal repair of spina bifida aperta
    Article Snippet: 150–300 ng of total RNA were reverse transcribed into cDNA with the Whole Transcriptome Amplification Kit (WTA2, Merck/Sigma-Aldrich) and purified with NucleoSpin Gel and PCR Clean-up (740611.50; Macherey-Nagel, Switzerland). .. Pre-amplification with the Qiagen Multiplex PCR Plus Kit (206152) of the cDNA was conducted. .. Gene expression analysis of NR3C1 and FKBP5 was performed in triplicate (1 μL PreAmp cDNA) using QuantiNova SYBR Green detection (Qiagen, Hombrechtikon, Switzerland) and normalized with five reference genes ( RPL13a , PPIA , GAPDH , 18S , ACTB ).

    Article Title: Signals of corresponding genetic diversity loss in four warbler species exhibiting regional or range-wide declines.
    Article Snippet: .. First round PCR was performed using the Qiagen Multiplex PCR Plus Kit (206152) with 0.3ul of the above primer mix and 2 - 50 ng of DNA in a final volume of 11ul. ..

    Article Title: Signals of corresponding genetic diversity loss in four warbler species exhibiting regional or range-wide declines
    Article Snippet: .. First round PCR was performed using the Qiagen Multiplex PCR Plus Kit (206,152) with 0.3 μl of the above primer mix and 2–50 ng of DNA in a final volume of 11 μl. .. Initial denaturation at 95 °C for 5 min was followed with 30 cycles of 95 °C for 30 s, 62 °C for 90 s, and 72 °C for 30 s, and a final extension step of 68 °C for 10 min. A tenfold dilution of these products was used as the template for a second round of PCR to add the Nextera i5 and i7 index primers.

    Article Title: Optimal Environmental DNA Sampling Strategy for Comprehensively Assessing Biodiversity in Rice Paddy Fields
    Article Snippet: In the 1st PCR, the 5′ termini of the above primers had Illumina Rd1 and Rd2 sequences. .. PCR with fwhF2/EPTDr2n used the multiplex PCR Plus kit (Qiagen), with a thermal profile of 95°C for 5 min; a touchdown of 10 cycles of 95°C for 30 s, 64 to 55°C (−1°C each cycle) for 90 s, and 72°C for 30 s; 25 cycles of 95°C for 30 s, 54°C for 90 s, and 72°C for 30 s; and a final 68°C for 10 min. PCR with MiFish- U and MiBird- U used KAPA HiFi Ready Mix HS FIGURE 1 | Study paddies and eDNA sampling positions. (A) Location of paddies (pale blue rectangles, A–G). ..

    Article Title: Food poisoning from raw horse meat contaminated with Shiga toxin-producing Escherichia coli O157 linked to nationwide spread of closely related strains, Japan, 2023
    Article Snippet: The PCR conditions were as follows: 95°C for 2 min; 35 cycles of 95°C for 30 s, 60°C for 90 s, and 72°C for 60 s; with final extension at 72°C for 10 min. PCR products were separated using a SeqStudio Genetic Analyzer (ThermoFisher Scientific). .. At the NIID, the Qiagen Multiplex PCR Plus Kit (Qiagen) was also used for PCR amplification, and an Applied Biosystems 3500xl Genetic Analyzer (ThermoFisher Scientific) was used for the separation of PCR products. ..

    Article Title: Food poisoning from raw horse meat contaminated with Shiga toxin-producing Escherichia coli O157 linked to nationwide spread of closely related strains, Japan, 2023.
    Article Snippet: The PCR conditions were as follows: 95°C for 2 min; 35 cycles of 95°C for 30 s, 60°C for 90 s, and 72°C for 60 s; with final extension at 72°C for 10 min. PCR products were separated using a SeqStudio Genetic Analyzer (ThermoFisher Scientific). .. At the NIID, the Qiagen Multiplex PCR Plus Kit (Qiagen) was also used for PCR amplification, and an Applied Biosystems 3500xl Genetic Analyzer (ThermoFisher Scientific) was used for the separation of PCR products. ..

    Article Title: Novel Y-STRs with elevated mutation rates further improve male relative differentiation.
    Article Snippet: .. PCRs were performed using the Qiagen Multiplex PCR Plus Kit with the following reaction mix: 5 μL Multiplex PCR Master Mix (2 ×), 1 μL primer mix (forward and reverse primers, 10 μM each), 2 μL Q- Solution, 1 μL RNase-free water, and 1 μL template DNA (10 ng/μL). ..

    Sampling:

    Article Title: Optimal Environmental DNA Sampling Strategy for Comprehensively Assessing Biodiversity in Rice Paddy Fields
    Article Snippet: In the 1st PCR, the 5′ termini of the above primers had Illumina Rd1 and Rd2 sequences. .. PCR with fwhF2/EPTDr2n used the multiplex PCR Plus kit (Qiagen), with a thermal profile of 95°C for 5 min; a touchdown of 10 cycles of 95°C for 30 s, 64 to 55°C (−1°C each cycle) for 90 s, and 72°C for 30 s; 25 cycles of 95°C for 30 s, 54°C for 90 s, and 72°C for 30 s; and a final 68°C for 10 min. PCR with MiFish- U and MiBird- U used KAPA HiFi Ready Mix HS FIGURE 1 | Study paddies and eDNA sampling positions. (A) Location of paddies (pale blue rectangles, A–G). ..

    Amplification:

    Article Title: Food poisoning from raw horse meat contaminated with Shiga toxin-producing Escherichia coli O157 linked to nationwide spread of closely related strains, Japan, 2023
    Article Snippet: The PCR conditions were as follows: 95°C for 2 min; 35 cycles of 95°C for 30 s, 60°C for 90 s, and 72°C for 60 s; with final extension at 72°C for 10 min. PCR products were separated using a SeqStudio Genetic Analyzer (ThermoFisher Scientific). .. At the NIID, the Qiagen Multiplex PCR Plus Kit (Qiagen) was also used for PCR amplification, and an Applied Biosystems 3500xl Genetic Analyzer (ThermoFisher Scientific) was used for the separation of PCR products. ..

    Article Title: Food poisoning from raw horse meat contaminated with Shiga toxin-producing Escherichia coli O157 linked to nationwide spread of closely related strains, Japan, 2023.
    Article Snippet: The PCR conditions were as follows: 95°C for 2 min; 35 cycles of 95°C for 30 s, 60°C for 90 s, and 72°C for 60 s; with final extension at 72°C for 10 min. PCR products were separated using a SeqStudio Genetic Analyzer (ThermoFisher Scientific). .. At the NIID, the Qiagen Multiplex PCR Plus Kit (Qiagen) was also used for PCR amplification, and an Applied Biosystems 3500xl Genetic Analyzer (ThermoFisher Scientific) was used for the separation of PCR products. ..



    Similar Products

    94
    Qiagen 2x qiagen multiplex pcr plus master mix
    2x Qiagen Multiplex Pcr Plus Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+plus+kit/QIAGEN+Multiplex+PCR+Plus+Kit+x+%E2%80%8B%E2%80%8B100u/pmc13639227-78-6-7
    Average 94 stars, based on 1 article reviews
    2x qiagen multiplex pcr plus master mix - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    99
    Qiagen multiplex pcr plus kit
    Multiplex Pcr Plus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+plus+kit/QIAGEN+Multiplex+PCR+Kit/pmc13141936-54-5-9
    Average 99 stars, based on 1 article reviews
    multiplex pcr plus kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Qiagen qiagen multiplex pcr plus kit
    Qiagen Multiplex Pcr Plus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+plus+kit/QIAGEN+Multiplex+PCR+Kit/pmc13125950-132-14-19
    Average 99 stars, based on 1 article reviews
    qiagen multiplex pcr plus kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Qiagen pcr multiplex plus kit
    Pcr Multiplex Plus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+plus+kit/QIAGEN+Multiplex+PCR+Kit/pmc13090109-85-6-10
    Average 99 stars, based on 1 article reviews
    pcr multiplex plus kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Qiagen multiplex plus pcr kit
    Multiplex Plus Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+plus+kit/QIAGEN+Multiplex+PCR+Kit/10__12972_slash_jabng__2026__10__1__2-33-5-9
    Average 99 stars, based on 1 article reviews
    multiplex plus pcr kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results